TABLE OF CONTENTS

APPENDIX Q. PHYSICAL AND BIOLOGICAL CONTAINMENT FOR RECOMBINANT DNA RESEARCH INVOLVING ANIMALS 1

Appendix Q-I. General Considerations 2

Appendix Q-I-A. Containment Levels 2

Appendix Q-I-B. Disposal of Animals (BL1-N through BL4-N) 3

Appendix Q-II. Physical and Biological Containment Levels 3

Appendix Q-II-A. Biosafety Level 1 - Animals (BL1-N) 3

Appendix Q-II-A-1. Standard Practices (BL1-N) 3

Appendix Q-II-A-1-a. Animal Facility Access (BL1-N) 3

Appendix Q-II-A-1-b. Other (BL1-N) 3

Appendix Q-II-A-2. Animal Facilities (BL1-N) 3

Appendix Q-II-B. Biosafety Level 2 - Animals (BL2-N) (See Appendix Q-III-A, Footnotes and References for Appendix Q) 3

Appendix Q-II-B-1. Standard Practices (BL2-N) 3

Appendix Q-II-B-1-a. Animal Facility Access (BL2-N) 3

Appendix Q-II-B-1-b. Decontamination and Inactivation (BL2-N) 4

Appendix Q-II-B-1-c. Signs (BL2-N) 4

Appendix Q-II-B-1-d. Protective Clothing (BL2-N) 4

Appendix Q-II-B-1-e. Records (BL2-N) 4

Appendix Q-II-B-1-f. Transfer of Materials (BL2-N) 4

Appendix Q-II-B-1-g. Other (BL2-N) 5

Appendix Q-II-B-2. Animal Facilities (BL2-N) 5

Appendix Q-II-C. Biosafety Level 3 - Animals (BL3-N) (See Appendix Q-III-B, Footnotes and References for Appendix Q) 6

Appendix Q-II-C-1. Standard Practices (BL3-N) 6

Appendix Q-II-C-1-a. Animal Facility Access (BL3-N) 6

Appendix Q-II-C-1-b. Decontamination and Inactivation (BL3-N) 6

Appendix Q-II-C-1-c. Signs (BL3-N) 6

Appendix Q-II-C-1-d. Protective Clothing (BL3-N) 6

Appendix Q-II-C-1-e. Records (BL3-N) 7

Appendix Q-II-C-1-f. Transfer of Materials (BL3-N) 7

Appendix Q-II-C-1-g. Other (BL3-N) 7

Appendix Q-II-C-2. Animal Facilities (BL3-N) 8

Appendix Q-II-D. Biosafety Level 4 - Animals (BL4-N) (See Appendix Q-III-C, Footnotes and References for Appendix Q) 9

Appendix Q-II-D-1. Standard Practices (BL4-N) 9

Appendix Q-II-D-1-a. Animal Facility Access (BL4-N) 9

Appendix Q-II-D-1-b. Decontamination and Inactivation (BL4-N) 10

Appendix Q-II-D-1-c. Signs (BL4-N) 11

Appendix Q-II-D-1-d. Protective Clothing (BL4-N) 11

Appendix Q-II-D-1-e. Records (BL4-N)